Liquid nitrogen free and cryogen free controlled rate freezer for research into the
cryopreservation of a wide range of material including: embryos, stem cells, mammalian cells,
spermatozoa, antibodies, tissue sections and rodent organs. The CRF-1 brings accuracy,
precision and reproducibility to biological cryopreservation.
Unlike conventional liquid nitrogen based controlled rate cooling equipment, the CRF-1 greatly reduces the risk
of contamination and can be used in cleanrooms and barrier facilities. The CRF-1 fits neatly on a bench-top. Its
performance in terms of cell viability after freezing is comparable or better than standard liquid nitrogen freezers. As
alcohol is not used, there is also a greatly reduced fire risk. The CRF-1 will cool down to -100ￜￜC with straws.
The cooling rate of the CRF-1 is precisely controlled, ensuring accuracy and reproducibility throughout the freezing
profile, especially for the important nucleation/seeding phase. This ensures optimal recovery of cells upon thawing.
Operation is simple and can be carried out with or without a PC; data can be logged via PC software and cooling
profiles are directly displayed on PC screen. Different cooling profiles are available from a drop down menu and
customised profiles can be written.
Key Features
 Accurate and reproducible control of cooling rates and sample temperatures
Easy to use and samples can be nucleated/seeded in-situ
Linear and non-linear cooling profiles
Low running costs: estimated at 1% of liquid nitrogen controlled rate freezing
Temperature remains at -100ￜￜC at the end of cycle for straw applications until freezer is switched off
Uninterruptible Power Supply (UPS): complete cycle run if power fails (supplied as an optional accessory)
CE marked (laboratory use)
Servicing and calibration available
3 year warranty
Data Sheet
Manual
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Applications
The CRF-1 is highly versatile and can be used for the cryopreservation research of a wide range of samples in
cryovials, straws, bags, microplates and Matrix-96-well block plates in the following areas:
Transgenic embryos research
Stem cell research
Clinical and research samples, e.g. lymphocytes and tissue cell lines in conventional cryovials
Various mammalian cells including cardiomyocytes, adipose, liver and muscle
Cord blood derived stem cells
Adherent cells and stem cells in microplates
Cell suspensions in numbered/barcoded arrays
Robotic integration - the CRF-1 has also successfully been integrated into robotic systems
Is also suitable for applications in veterinarian IVF
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